Cytochrome b gene has a genetic content preserved between animals and it is used
especially for the vertebrate’s identification. In the forensic context it is specially used
to determine the origin of non human samples. The sequencing strategy that was
implemented in our laboratory, BigDey/BetterBuffer/XTerminator, allowed to reduce
time procedures, improve quality of data and reduce significantly the cost per reaction
comparing to other described methodologies. Using the program BLAST the obtained
sequence is aligned and compared with sequences of the cytochrome b gene registered
in a database (http://blast.ncbi.nlm.nih.gov/Blast.cgi). The aim of this study is the
identification of several vertebrate animals to allow the resolution of eventual forensic
cases in a shorter time than traditional methodologies and with lower costs