As analysis of mtDNA hypervariable regions is a laborious technique, it is desirable
to type mtSN Ps before performing sequencing. This study presents a 10 SN P multiplex,
selected from mitochondrial coding region loci. These markers can subdivide any
population samples in H* and non-H* haplogroups, assigning non-H* haplogroups,
which in same cases is useful for forensic casework. The SN P multiplex detected 13
different non-H* haplogroups encountered in the two most common populations in our
casework – European and African ancestry populations. Haplogroups U, J, T and L1/
L2 are the most frequent non-H* haplogroups in the Portuguese population samples.
A probable new variant was assigned as a U/K haplogroup. This coding SN P assay is a
simple method and can increase the discrimination of hypervariable region haploypes